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The PCR conditions were: 94 C for 5 min

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Immunofluorescence MCF7 and MDA-MB-231 cells were seeded on coverslips in a 12-well plate at a density of 10,000 cells per well and allowed to adhere overnight

Despite more than 500 published preclinical studies (primarily animal and cell-culture models from Sikiric and colleagues at the University of Zagreb), there have been essentially no registered human clinical trials for BPC-157 in the United States